Tinción, Tinciones especiales
Grocott – kit – 1 Kit – 100 Test
Kit de siete reactivos para la tinción de hongos
Introducción
El Kit Grocott se utiliza en histología para la visualización de hongos, ciertos patógenos, membranas basales y estructuras de argentafina en general. El procedimiento de tinción comienza con una solución ácida periódica que se utiliza para oxidar los 1,2-glicoles a aldehídos. Durante la incubación en la solución de trabajo de metenamina-borato de plata, los aldehídos se reducen y al mismo tiempo provocan la reducción de los iones de plata a plata metálica que se manifiesta como estructuras negras en la sección. A continuación, se tonifica la solución con una solución de cloruro de oro que, además, mejora la intensidad de la tinción de las estructuras objetivo (hongos, membranas basales y otras) y reduce la tinción de fondo. El exceso de enlaces plataoro no unidos se elimina enjuagando la sección con una solución de tiosulfato de sodio. Por último, las secciones se exponen al colorante Fast Green F.C.F. que tiñe las estructuras de fondo; eso, a su vez, crea un contraste claro y de alcance visual con las estructuras objetivo (coloreadas de marrón a negro).
Resultados
Membranas basales, glucógeno, bacterias y hongos – marrón a negro
Fondo – verde
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