Tinción, Tinciones especiales
Gram – kit – 1 Kit – 100 Test
Kit de cinco reactivos para la identificación de bacterias según Gram en secciones histológicas
Para diferenciar las bacterias Gram positivas de las Gram negativas
La tinción de Gram es un método para diferenciar especies bacterianas y es comúnmente conocido y utilizado en microbiología. También es uno de los métodos de diagnóstico más utilizados en los laboratorios clínicos y hospitalarios. La tinción de Gram diferencia las bacterias en dos grupos: Gram-positivas y Gram-negativas.
Esta división se basa en las diferencias estructurales de las membranas bacterianas de ambos grupos, es decir, su capacidad para retener el colorante. Las bacterias Gram-positivas tienen una membrana celular más gruesa que permite retener el colorante dentro de la célula al tratarlas con una solución de yodo que crea un complejo insoluble de yodo y colorante primario. Las bacterias Gram negativas tienen una estructura de membrana celular más fina que no puede retener el colorante. Éste se desprende a través de la membrana y el uso de la contratinción constituye la base para diferenciar entre estos dos grupos de bacterias. El Kit Histo BioGram, contiene solución de Gram Crystal Violet al 1%, solución estabilizada de Gram Lugol, dos paquetes de solución de Gram Decolorizer 2,
solución de Gram Safranin y dos paquetes de ácido pícrico en acetona. Sus características lo convierten en un óptimo agente de tinción de bacterias que proporciona resultados consistentes.
Resultados
Bacterias Gram-positivas – azul-púrpura
Bacterias Gram-negativas – rojo
Coloración de fondo – tonos de púrpura-rosa
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