Tinción, Tinciones especiales
P.A.S.M. / Jones – Plata metenamina – kit – 1 Kit – 100 Test
Kit de ácido periódico-plata-metenamina para la tinción de estructuras de argentafina y membranas renales.
El Kit P.A.S.M./Jones se utiliza en histología para visualizar estructuras argentafínicas, especialmente membranas renales, pero también hongos y ciertos organismos patógenos. El procedimiento de tinción comienza con una solución ácida periódica que se utiliza para oxidar los 1,2-glicoles a aldehídos. Durante la incubación, en la solución de trabajo de plata-metenamina-borato, los aldehídos se reducen y al mismo tiempo causan la reducción de los iones de plata a plata metálica que se manifiesta como estructuras de color marrón a negro en la sección. A continuación, se tonifica la solución con una solución de cloruro de oro que mejora adicionalmente la intensidad de la tinción de las estructuras objetivo (hongos, membranas basales y otras) y reduce la tinción de fondo. El exceso de enlaces plata-oro no unidos se elimina enjuagando la sección con una solución de tiosulfato de sodio. Por último, las secciones se exponen a la contratinción con el reactivo Nuclear Fast Red (Kernechtrot) que tiñe de rojo las estructuras de fondo, lo que a su vez crea un contraste claro y visualmente rico con las estructuras objetivo (coloreadas en marrón-negro).
Resultados
Membranas basales, glucógeno, bacterias y hongos – marrón a negro
Fondo – rosa
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